Re: Abbott

On Fri, 18 Oct 2024 21:11:32 -0000 (UTC), Tom Kunich
<cyclintom@yahoo.com> wrote:

Why chemically based insulin is so
difficult to make that anyone could make it in a reasonably equiped
laboratory but..."

Anyone could make it?

"IWTL: How to make insulin in a small home laboratory"  (2010)
<https://www.reddit.com/r/askscience/comments/e5drj/iwtl_how_to_make_insulin_in_a_small_home/>
"Insulin is a protein, thus it can be synthesized by adding individual
amino acids, which in any case, would be a pain at best. Following the
correct amino acid sequence, you must have it fold correctly, and that
is a lot easier said than done. So hard that companies have given up
producing insulin this way and the commercial way to do it is to grow
genetically modified bacteria that can produce Insulin."
(...)
"Even if you do get this far, the challenge continues. How do you
extract the insulin from the bacteria? You could design them to
secrete it into the surrounding growth solution, but then you would
have to attach a sequence to the beginning that tells the bacteria to
export the protein. A hard obstacle to overcome from what I know, as
there must be other specific proteins to stabilize the unfolded
insulin before export."


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Jeff Liebermann                 jeffl@cruzio.com
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