Re: Abbott

On Fri, 18 Oct 2024 15:24:23 -0700, Jeff Liebermann wrote:

On Fri, 18 Oct 2024 21:11:32 -0000 (UTC), Tom Kunich
<cyclintom@yahoo.com> wrote:
 

Why chemically based insulin is so difficult to make that anyone could
make it in a reasonably equiped laboratory but..."

 
Anyone could make it?
 
"IWTL: How to make insulin in a small home laboratory"  (2010)
<https://www.reddit.com/r/askscience/comments/e5drj/

iwtl_how_to_make_insulin_in_a_small_home/>

"Insulin is a protein, thus it can be synthesized by adding individual
amino acids, which in any case, would be a pain at best. Following the
correct amino acid sequence, you must have it fold correctly, and that
is a lot easier said than done. So hard that companies have given up
producing insulin this way and the commercial way to do it is to grow
genetically modified bacteria that can produce Insulin."
(...)
"Even if you do get this far, the challenge continues. How do you
extract the insulin from the bacteria? You could design them to secrete
it into the surrounding growth solution, but then you would have to
attach a sequence to the beginning that tells the bacteria to export the
protein. A hard obstacle to overcome from what I know, as there must be
other specific proteins to stabilize the unfolded insulin before
export."

More knowledge from the man on welfare. Insulin can be made strictly by
chemistry. Bacterial production is for mass production and has strict
limmits since it is then very difficult to purify. But being the
biological whizz kid you already knew that before you attempted to impress
everyone with your use of Google.